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In sexual recombination, new combinations of genes are created by which of the following processes?


A) Transduction
B) Crossing over
C) Conjugation
D) Transformation

E) None of the above
F) All of the above

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In a DNA molecule, one end has a free hydroxyl group and one end has a free ___________.


A) phosphate group
B) hydroxyl group
C) amino group
D) carboxyl group

E) B) and C)
F) A) and D)

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What type of bonds spontaneously form between two complementary strands of DNA?


A) Hydrogen bonds
B) Covalent bonds
C) Phosphodiester bonds
D) Polar covalent bonds

E) B) and C)
F) A) and B)

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Which of the following is a type II restriction enzyme recognition site?


A) GAATTC
B) GAATTG
C) GAAAAG
D) GATCAG

E) A) and C)
F) All of the above

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The lagging DNA strand is synthesized discontinuously producing:


A) Kornberg fragments
B) Southern fragments
C) Okazaki fragments
D) Klenow fragments

E) All of the above
F) A) and B)

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Enzymes that recognize palindromic sequences of DNA, that are cut within the recognition sequence, that do not have methylating activity, and that are used frequently in the laboratory are which type of restriction enzymes?


A) Type I
B) Type II
C) Type III
D) Type IV

E) All of the above
F) A) and C)

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In DNA replication, the leading strand is the strand that has which orientation?


A) 5to 3
B) 3to 5
C) Both strands are leading.

D) All of the above
E) A) and B)

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DNA replication is:


A) Conservative
B) Semiconservative
C) Nonconservative
D) Dispersive

E) A) and D)
F) A) and B)

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B

Avery, MacLeod, and McCarty identified the nature of Griffith's transforming factor in an experiment where it was destroyed by what treatment?


A) Protease
B) Ribonuclease
C) Deoxyribonuclease
D) Heating

E) All of the above
F) B) and C)

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What is the fertility factor (F) observed by Hayes and Lederberg?


A) A chemical that encourages bacterial mating
B) A virus
C) A plasmid
D) A polysaccharide on the cell surface

E) A) and D)
F) All of the above

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Nucleotides in DNA consist of which of the following?


A) Nitrogen base, deoxyribose, and phosphate
B) Nitrogen base, ribose, and sulfur
C) Carbon base, ribose, and phosphate
D) Carbon base, glucose, and carboxyl

E) A) and B)
F) B) and D)

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DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond?


A) Aldehyde
B) Ester
C) Phosphodiester
D) Peptide

E) A) and D)
F) A) and C)

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C

Which of the following promote resistance to common antibiotics?


A) Helicases
B) R factors
C) Endonucleases
D) Methylases

E) A) and B)
F) A) and C)

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DNA replication proceeds with the new daughter strand synthesized in the following orientation:


A) 5to 3
B) 3to 5
C) The daughter strand is not synthesized in any order.
D) Either 5to 3or 3 to 5

E) B) and C)
F) A) and D)

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The purpose of ligase is to:


A) Synthesize DNA
B) Cut DNA within the double helix
C) Chew DNA from the ends
D) Paste two ends of DNA together

E) All of the above
F) None of the above

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Which of the following does not play a role in DNA replication?


A) RNA primer
B) Deoxynucleotide
C) Stop codon
D) DNA polymerase

E) A) and B)
F) None of the above

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Which DNA sequence is complementary to 5GATTCTCAAAGGACT3?


A) 5GATTCTCAAAGGACT3
B) 3GATTCTCAAAGGACT5
C) 3CTAAGAGTTTCCTGA5
D) 5CTAAGAGTTTCCTGA3

E) A) and B)
F) A) and D)

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In a double-stranded DNA molecule, base pairing between strands occurs between:


A) A purine and a purine
B) A pyrimidine and a pyrimidine
C) A purine and a pyrimidine
D) Any type of nucleotide base

E) B) and C)
F) A) and D)

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What reactions are catalyzed by DNA methyltransferase?


A) Addition of methyl groups to nitrogen bases
B) Addition of methyl groups to ribose sugars
C) Addition of methyl groups to proteins
D) Removal of methyl groups from DNA

E) B) and D)
F) C) and D)

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A

In DNA replication, where does replication begin and end?


A) Origin of replication; end of the molecule
B) Promoter; termination site
C) Start codon; stop codon
D) Start codon, termination site

E) All of the above
F) B) and C)

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